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CooperSurgical sage ivm medium
Sage Ivm Medium, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sage+ivm+medium/sage+ivm+medium/pm40447125-89-125-141
Average 90 stars, based on 1 article reviews
sage ivm medium - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: The effect of short-term exposure of cumulus-oocyte complexes to in vitro maturation medium on yield of mature oocytes and usable embryos in stimulated cycles
Article Snippet: .. Short-term exposure to IVM medium (i.e., in cycle 2 of the treated group) consisted of the intact COCs being placed in SAGE IVM medium (Cooper Surgical, Trumbull, CT, USA) immediately after retrieval and subsequently incubated for 3–5 h [ 9 ]. ..

other:

Article Title: Fertility preservation strategies for cancerous women: An updated review
Article Snippet: Commercial IVM media, such as SAGE (Cooper surgical) IVM medium, MediCult IVM medium (MediCult, Origio, Måløv, Denmark) ( ) , and tissue culture medium 199 (TCM199, Invitrogen, Carlsbad, CA) ( ) have the advantage of immature oocytes culture.

Article Title: Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries.
Article Snippet: Recently, commercialized IVM media such as SAGE (Coopersurgical) IVM medium and MediCult IVM medium have been used in several IVF centers with an advantage that is certified as IVF quality controlled.

Article Title: Immature Oocyte for Fertility Preservation
Article Snippet: Recently, commercialized IVM media such as SAGE (Coopersurgical) IVM medium and Medi-Cult IVM medium have been used in several IVF centers as they have the advantage of being certified as IVF quality controlled ( – ).

Article Title: Rescue in vitro maturation of germinal vesicle oocytes after ovarian stimulation: the importance of the culture media.
Article Snippet: The media tested and reported in the literature across the years are listed and described in Table 1: B2 medium (Janssenswillen et al., 1995; Nogueira et al., 2000; Torre et al., 2006), HTF medium (human tubal fluid; Edirisinghe et al., 1997; Farhi et al., 1997; Chen et al., 2000; Escrich et al., 2011; Lee et al., 2016), M-199 medium (Goud et al., 1998; Vanhoutte et al., 2007; Li et al., 2019), Earle’s medium (Haberle et al., 1999), TLP medium (Kim et al., 2000), TCM-199 medium (Chian and Tan, 2002; Roberts et al., 2002; Combelles et al., 2005), MEME medium (Roberts et al., 2002), Ham’s F10 medium (Fesahat et al., 2017; Chatroudi et al., 2019), and commercialized media for standard IVM like SAGE (CooperSurgical, CT, USA) IVM medium (Reichman et al., 2010; Fesahat et al., 2017; Kasapi et al., 2017), and MediCult (CooperSurgical) IVM System (Al-Khtib et al., 2011; Virant-Klun et al., 2018).

Cell Culture:

Article Title: Bisphenol-A and human oocyte maturation in vitro
Article Snippet: .. Culture system and preparation of BPA solutions Oocytes were cultured for 30 h at 37°C in a humidified atmosphere of 5% CO in air in 8-well glass slides (LabTek, #1256518, No. 177402; Thermo Fisher Scientific, USA) containing 300 μl of SAGE IVM medium (CooperSurgical, Trumbull, CT, USA) with 0, 20, 200 ng/ml or 20 μg/ml BPA (CAS Number: 80-05-7; Sigma-Aldrich). ..



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CooperSurgical sage ivm medium
Sage Ivm Medium, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sage+ivm+medium/sage+ivm+medium/pm40447125-89-125-141
Average 90 stars, based on 1 article reviews
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CooperSurgical conventional ivm medium sage
Egg retrieval process in mouse for <t>IVM.</t> I. First ovaries were obtained from stimulated mice. II. Using the stereo microscope ovary was scratched. III. Each individual GV oocyte was recovered and IV. Then cultured separately in a small microdrop for IVM. The GV oocyte within each drop was coded for the follow up and data analysis. IVM progress steps at different times in hTCCM and control and sham groups. After 8, 16 and 24 hours in hTCCM and sham condition, some of the GV oocytes (A, I) developed further and become mature MII oocytes (B-D, K-L). Nevertheless, in DMEM + 20% FBS condition GV oocytes (E) could develop to MI oocytes (F-H) but none of them could complete the IVM process to form MII oocytes. IVM; In vitro maturation, GV; Germinal vesicle, hTCCM; Human testicular cell conditioned medium, MII; Metaphase II, DMEM; Dulbecco's Modified Eagle Medium, FBS; Fetal bovine serum, and H; Hours.
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Merck KGaA sage ivm maturation medium with mg-132
Egg retrieval process in mouse for <t>IVM.</t> I. First ovaries were obtained from stimulated mice. II. Using the stereo microscope ovary was scratched. III. Each individual GV oocyte was recovered and IV. Then cultured separately in a small microdrop for IVM. The GV oocyte within each drop was coded for the follow up and data analysis. IVM progress steps at different times in hTCCM and control and sham groups. After 8, 16 and 24 hours in hTCCM and sham condition, some of the GV oocytes (A, I) developed further and become mature MII oocytes (B-D, K-L). Nevertheless, in DMEM + 20% FBS condition GV oocytes (E) could develop to MI oocytes (F-H) but none of them could complete the IVM process to form MII oocytes. IVM; In vitro maturation, GV; Germinal vesicle, hTCCM; Human testicular cell conditioned medium, MII; Metaphase II, DMEM; Dulbecco's Modified Eagle Medium, FBS; Fetal bovine serum, and H; Hours.
Sage Ivm Maturation Medium With Mg 132, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CooperSurgical sage ivm washing medium
Techniques for transporting human follicular fluid. A, Appearance of the 300‐mL vacuum‐insulated bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage <t>IVM</t> <t>maturation</t> medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®
Sage Ivm Washing Medium, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sage+ivm+medium/sage+ivm+medium/pmc06452027-66-16-20
Average 90 stars, based on 1 article reviews
sage ivm washing medium - by Bioz Stars, 2026-09
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CooperSurgical sage ivm maturation medium
Techniques for transporting human follicular fluid. A, Appearance of the <t>300‐mL</t> <t>vacuum‐insulated</t> bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage <t>IVM</t> maturation medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®
Sage Ivm Maturation Medium, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sage+ivm+medium/sage+ivm+medium/pmc06452027-65-16-20
Average 90 stars, based on 1 article reviews
sage ivm maturation medium - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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Egg retrieval process in mouse for IVM. I. First ovaries were obtained from stimulated mice. II. Using the stereo microscope ovary was scratched. III. Each individual GV oocyte was recovered and IV. Then cultured separately in a small microdrop for IVM. The GV oocyte within each drop was coded for the follow up and data analysis. IVM progress steps at different times in hTCCM and control and sham groups. After 8, 16 and 24 hours in hTCCM and sham condition, some of the GV oocytes (A, I) developed further and become mature MII oocytes (B-D, K-L). Nevertheless, in DMEM + 20% FBS condition GV oocytes (E) could develop to MI oocytes (F-H) but none of them could complete the IVM process to form MII oocytes. IVM; In vitro maturation, GV; Germinal vesicle, hTCCM; Human testicular cell conditioned medium, MII; Metaphase II, DMEM; Dulbecco's Modified Eagle Medium, FBS; Fetal bovine serum, and H; Hours.

Journal: International Journal of Fertility & Sterility

Article Title: Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes

doi: 10.22074/ijfs.2020.6097

Figure Lengend Snippet: Egg retrieval process in mouse for IVM. I. First ovaries were obtained from stimulated mice. II. Using the stereo microscope ovary was scratched. III. Each individual GV oocyte was recovered and IV. Then cultured separately in a small microdrop for IVM. The GV oocyte within each drop was coded for the follow up and data analysis. IVM progress steps at different times in hTCCM and control and sham groups. After 8, 16 and 24 hours in hTCCM and sham condition, some of the GV oocytes (A, I) developed further and become mature MII oocytes (B-D, K-L). Nevertheless, in DMEM + 20% FBS condition GV oocytes (E) could develop to MI oocytes (F-H) but none of them could complete the IVM process to form MII oocytes. IVM; In vitro maturation, GV; Germinal vesicle, hTCCM; Human testicular cell conditioned medium, MII; Metaphase II, DMEM; Dulbecco's Modified Eagle Medium, FBS; Fetal bovine serum, and H; Hours.

Article Snippet: Human TCs are cultured in DMEM + 20% FBS, thereby, for preparation of hTCCM, the conventional IVM medium (Sage; Cooper Surgical) and recently routine culture medium for IVM (TCM-199; Sigma, USA) were not used in our study.

Techniques: Microscopy, Cell Culture, Control, In Vitro, Modification

Rate of the degeneration, MI and MII oocytes formation in hTCCM, DMEM + 20% FBS and Ham’s F10 + HFF media after 8, 16 and 24 hours. A. Rate of the degeneration increased in three media during 24 hours. The highest rate of the degeneration was in the control medium. B. In hTCCM group and Ham’s F10 + HFF medium the number of MI oocytes decreased after 16 and 24 hours due to further maturation to MII oocytes. In the control group MI oocytes decreased after 16 hours due to degeneration. In the control group none of the GV oocytes developed to MII stage. C. hTCCM and Ham’s F10 + HFF medium seem to have a supportive progressive effect on the IVM rate to MII stage after 8, 16 and 24 hours. IVM; In vitro maturation, GV; Germinal vesicle, hTCCM; Human testicular cell conditioned medium, MI; Metaphase I, MII; Metaphase II, DMEM; Dulbecco's Modified Eagle Medium, FBS; Fetal bovine serum, HFF; Human follicle fluid, and H; Hours.

Journal: International Journal of Fertility & Sterility

Article Title: Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes

doi: 10.22074/ijfs.2020.6097

Figure Lengend Snippet: Rate of the degeneration, MI and MII oocytes formation in hTCCM, DMEM + 20% FBS and Ham’s F10 + HFF media after 8, 16 and 24 hours. A. Rate of the degeneration increased in three media during 24 hours. The highest rate of the degeneration was in the control medium. B. In hTCCM group and Ham’s F10 + HFF medium the number of MI oocytes decreased after 16 and 24 hours due to further maturation to MII oocytes. In the control group MI oocytes decreased after 16 hours due to degeneration. In the control group none of the GV oocytes developed to MII stage. C. hTCCM and Ham’s F10 + HFF medium seem to have a supportive progressive effect on the IVM rate to MII stage after 8, 16 and 24 hours. IVM; In vitro maturation, GV; Germinal vesicle, hTCCM; Human testicular cell conditioned medium, MI; Metaphase I, MII; Metaphase II, DMEM; Dulbecco's Modified Eagle Medium, FBS; Fetal bovine serum, HFF; Human follicle fluid, and H; Hours.

Article Snippet: Human TCs are cultured in DMEM + 20% FBS, thereby, for preparation of hTCCM, the conventional IVM medium (Sage; Cooper Surgical) and recently routine culture medium for IVM (TCM-199; Sigma, USA) were not used in our study.

Techniques: Control, In Vitro, Modification

 IVM  rates at 8, 16, and 24 hours in the three groups

Journal: International Journal of Fertility & Sterility

Article Title: Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes

doi: 10.22074/ijfs.2020.6097

Figure Lengend Snippet: IVM rates at 8, 16, and 24 hours in the three groups

Article Snippet: Human TCs are cultured in DMEM + 20% FBS, thereby, for preparation of hTCCM, the conventional IVM medium (Sage; Cooper Surgical) and recently routine culture medium for IVM (TCM-199; Sigma, USA) were not used in our study.

Techniques:

The oocytes shape and PVS change rates in hTCCM and control media during IVM process. A. Normal GV, B. Normal MI, C. Normal MII, D, E. Wide PVS, F. Irregular shape, and G. Rate of wide PVS oocytes formation during IVM. In hTCCM and control groups the percent of wide PVS oocytes increased after 8, 16 and 24 hours. But this increasing is higher in hTCCM medium and different is significantly. H. Rate of irregular oocytes formation during IVM. In hTCCM and control groups the percent of irregular oocytes formation increased after 8, 16, 24 hours and there was significant difference between two groups. PVS; Perivitelline space, IVM; In vitro maturation, hTCCM; Human testicular cell conditioned medium, MI; Metaphase I, MII; Metaphase II, and H; Hours.

Journal: International Journal of Fertility & Sterility

Article Title: Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes

doi: 10.22074/ijfs.2020.6097

Figure Lengend Snippet: The oocytes shape and PVS change rates in hTCCM and control media during IVM process. A. Normal GV, B. Normal MI, C. Normal MII, D, E. Wide PVS, F. Irregular shape, and G. Rate of wide PVS oocytes formation during IVM. In hTCCM and control groups the percent of wide PVS oocytes increased after 8, 16 and 24 hours. But this increasing is higher in hTCCM medium and different is significantly. H. Rate of irregular oocytes formation during IVM. In hTCCM and control groups the percent of irregular oocytes formation increased after 8, 16, 24 hours and there was significant difference between two groups. PVS; Perivitelline space, IVM; In vitro maturation, hTCCM; Human testicular cell conditioned medium, MI; Metaphase I, MII; Metaphase II, and H; Hours.

Article Snippet: Human TCs are cultured in DMEM + 20% FBS, thereby, for preparation of hTCCM, the conventional IVM medium (Sage; Cooper Surgical) and recently routine culture medium for IVM (TCM-199; Sigma, USA) were not used in our study.

Techniques: Control, In Vitro

Rate of oocytes wide/normal PVS and irregular/normal shape following  IVM  in  hTCCM  and control group at different time points

Journal: International Journal of Fertility & Sterility

Article Title: Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes

doi: 10.22074/ijfs.2020.6097

Figure Lengend Snippet: Rate of oocytes wide/normal PVS and irregular/normal shape following IVM in hTCCM and control group at different time points

Article Snippet: Human TCs are cultured in DMEM + 20% FBS, thereby, for preparation of hTCCM, the conventional IVM medium (Sage; Cooper Surgical) and recently routine culture medium for IVM (TCM-199; Sigma, USA) were not used in our study.

Techniques: Control

Techniques for transporting human follicular fluid. A, Appearance of the 300‐mL vacuum‐insulated bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage IVM maturation medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Techniques for transporting human follicular fluid. A, Appearance of the 300‐mL vacuum‐insulated bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage IVM maturation medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®

Article Snippet: When HFF was transported under 5% CO 2 (as described below), it was mixed HFF with Sage IVM maturation medium (Cooper Surgical, Trumbull, CT, USA), which does not contain HEPES.

Techniques:

Collected oocytes from resected ovaries and immunofluorescent staining. A, Collected immature oocytes before in vitro maturation in patient 8. B, An oocyte in meiosis II showing the first polar body after in vitro maturation in patient 8. C, Immunofluorescent staining of a mature oocyte in patient 5. D, Immunofluorescent staining of a mature oocyte in patient 13. The spindle is enclosed in a square box. E, Enlarged view of the spindle corresponding to the boxed area in (D). White scale bar indicates 50 µm. Red scale bar indicates 25 µm. Green, microtubules; blue, DAPI

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Collected oocytes from resected ovaries and immunofluorescent staining. A, Collected immature oocytes before in vitro maturation in patient 8. B, An oocyte in meiosis II showing the first polar body after in vitro maturation in patient 8. C, Immunofluorescent staining of a mature oocyte in patient 5. D, Immunofluorescent staining of a mature oocyte in patient 13. The spindle is enclosed in a square box. E, Enlarged view of the spindle corresponding to the boxed area in (D). White scale bar indicates 50 µm. Red scale bar indicates 25 µm. Green, microtubules; blue, DAPI

Article Snippet: When HFF was transported under 5% CO 2 (as described below), it was mixed HFF with Sage IVM maturation medium (Cooper Surgical, Trumbull, CT, USA), which does not contain HEPES.

Techniques: Staining, In Vitro

Comparison of maturation rates in the transportation and non‐transportation groups. Non‐Transport, non‐transportation group; Bottle, bottle group; Box, box group. The bars indicate the percentages of oocytes that reached mature metaphase II as a result of in vitro maturation. Two‐sided Fisher's exact test was used for statistical analysis

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Comparison of maturation rates in the transportation and non‐transportation groups. Non‐Transport, non‐transportation group; Bottle, bottle group; Box, box group. The bars indicate the percentages of oocytes that reached mature metaphase II as a result of in vitro maturation. Two‐sided Fisher's exact test was used for statistical analysis

Article Snippet: When HFF was transported under 5% CO 2 (as described below), it was mixed HFF with Sage IVM maturation medium (Cooper Surgical, Trumbull, CT, USA), which does not contain HEPES.

Techniques: Comparison, In Vitro

Techniques for transporting human follicular fluid. A, Appearance of the 300‐mL vacuum‐insulated bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage IVM maturation medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Techniques for transporting human follicular fluid. A, Appearance of the 300‐mL vacuum‐insulated bottle and the Falcon conical tube. B, Falcon conical tube in a 300‐mL vacuum‐insulated bottle filled with warm water (37°C). C, CellBox ® and Thermopack ® . D, Appearance of CulturePal ® inside CellBox ® . E, Dish with HFF and Sage IVM maturation medium is contained in CulturePal ® . White bag shown by red arrow is the CulturePal ® CO 2 generator. F, Schema of Thermopack ® and CulturePal ® in CellBox ®

Article Snippet: Therefore, when HFF was transported in a vacuum‐insulated bottle (as described below), it was mixed with Sage IVM washing medium (Cooper Surgical) containing 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES).

Techniques:

Collected oocytes from resected ovaries and immunofluorescent staining. A, Collected immature oocytes before in vitro maturation in patient 8. B, An oocyte in meiosis II showing the first polar body after in vitro maturation in patient 8. C, Immunofluorescent staining of a mature oocyte in patient 5. D, Immunofluorescent staining of a mature oocyte in patient 13. The spindle is enclosed in a square box. E, Enlarged view of the spindle corresponding to the boxed area in (D). White scale bar indicates 50 µm. Red scale bar indicates 25 µm. Green, microtubules; blue, DAPI

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Collected oocytes from resected ovaries and immunofluorescent staining. A, Collected immature oocytes before in vitro maturation in patient 8. B, An oocyte in meiosis II showing the first polar body after in vitro maturation in patient 8. C, Immunofluorescent staining of a mature oocyte in patient 5. D, Immunofluorescent staining of a mature oocyte in patient 13. The spindle is enclosed in a square box. E, Enlarged view of the spindle corresponding to the boxed area in (D). White scale bar indicates 50 µm. Red scale bar indicates 25 µm. Green, microtubules; blue, DAPI

Article Snippet: Therefore, when HFF was transported in a vacuum‐insulated bottle (as described below), it was mixed with Sage IVM washing medium (Cooper Surgical) containing 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES).

Techniques: Staining, In Vitro

Comparison of maturation rates in the transportation and non‐transportation groups. Non‐Transport, non‐transportation group; Bottle, bottle group; Box, box group. The bars indicate the percentages of oocytes that reached mature metaphase II as a result of in vitro maturation. Two‐sided Fisher's exact test was used for statistical analysis

Journal: Reproductive Medicine and Biology

Article Title: Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non‐stimulated ovaries of patients with endometrial adenocarcinoma

doi: 10.1002/rmb2.12265

Figure Lengend Snippet: Comparison of maturation rates in the transportation and non‐transportation groups. Non‐Transport, non‐transportation group; Bottle, bottle group; Box, box group. The bars indicate the percentages of oocytes that reached mature metaphase II as a result of in vitro maturation. Two‐sided Fisher's exact test was used for statistical analysis

Article Snippet: Therefore, when HFF was transported in a vacuum‐insulated bottle (as described below), it was mixed with Sage IVM washing medium (Cooper Surgical) containing 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES).

Techniques: Comparison, In Vitro